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Goat Anti-U2AF1L4 Antibody (EB08161)

Related Products: EBP08161 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB08161 Goat Anti-U2AF1L4 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB08161 1
EB08161 (0.1µg/ml) staining of Human Cerebellum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: U2AF1L4, U2 small nuclear RNA auxiliary factor 1-like 4, FLJ35525, MGC33901, U2AF1-RS3, U2AF1L3, U2AF1L3V1, U2AF1RS3, U2af26, U2 small nuclear RNA auxiliary factor 1-like 3, U2(RNU2) small nuclear RNA auxiliary factor 1-like 3
Official Symbol: U2AF1L4
Accession Number(s): NP_001035515.1 ; NP_659424.2
Human GeneID(s): 199746
Non-Human GeneID(s): 233073 (mouse), 361542 (rat)
Important Comments: This antibody is expected to recognise both reported isoforms (NP_001035515.1 and NP_659424.2). No cross-reactivity with U2AF1 expected.

Immunogen

Peptide with sequence C-EDGERAVAELSNR, from the internal region of the protein sequence according to NP_001035515.1 ; NP_659424.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 20kDa band observed in Human Brain (Cerebellum) and Lung lysates (calculated MW of 21.3kDa according to NP_001035515.1). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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