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Goat Anti-UBR4 / ZUBR1 Antibody (EB09611)

Related Products: EBP09611 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB09611 Goat Anti-UBR4 / ZUBR1 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 13.30 PST on Monday through Thursday are shipped the same day.

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB09611 1
EB09611 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: UBR4, ubiquitin protein ligase E3 component n-recognin 4, FLJ41863, KIAA0462, KIAA1307, RBAF600, ZUBR1, p600, retinoblastoma-associated factor 600, retinoblastoma-associated factor 600-like protein, zinc finger, UBR1 type 1
Official Symbol: UBR4
Accession Number(s): NP_065816.2
Human GeneID(s): 23352
Non-Human GeneID(s): 69116 (mouse), 313658 (rat)

Immunogen

Peptide with sequence C-NTTRATSREEKNLQ, from the C Terminus of the protein sequence according to NP_065816.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 574kDa according to NP_065816.2. Therefore we cannot recommend an optimal concentration and the antibody is an aspiring product. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Spleen shows various types of subnuclear staining in select splenocytes and additional membrane plus cytoplasm staining elsewhere. Recommended concentration, 5-10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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