Change country
Home
Code Name Applications Availability Size Tested species Price Grade  
EB12494 Goat Anti-VPS16 (isoform 1) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

Ordering Information for

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline

99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

  • Product code(s)
  • Purchase order number
  • Delivery and invoice address
  • Contact telephone number and email address

In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB12494 1
EB12494 (0.1µg/ml) staining of HeLa (A), HepG2 (B) and Jurkat (C) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12494 2
EB12494 (0.1µg/ml) staining of Mouse Spleen lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12494 3
EB12494 (5µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: VPS16, vacuolar protein sorting 16 homolog (S. cerevisiae), hVPS16, OTTHUMP00000030047, OTTHUMP00000030048, vacuolar protein sorting protein 16, vacuolar protein sorting-associated protein 16 homolog
Official Symbol: VPS16
Accession Number(s): NP_072097.2
Human GeneID(s): 64601
Non-Human GeneID(s): 80743 (mouse), 296159 (rat)
Important Comments: This antibody is expected to recognize reported isoform 1 (NP_072097.2) only.

Immunogen

Peptide with sequence C-KESQKADEYLREIQ, from the internal region of the protein sequence according to NP_072097.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 100kDa band observed in lysates of cell lines HepG2, HeLa and Jurkat and in lysates of Mouse Spleen and Rat Thymus (calculated MW of 94.7kDa according to NP_072097.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Uterus. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Product Reviews

Please login to review this product.