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Goat Anti-VPS45 (C Terminus) Antibody (EB06145)

Related Products: EBP06145 - Immunizing Peptide, EBP06832 - Immunizing Peptide, Goat Anti-VPS45 (Internal) Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB06145 Goat Anti-VPS45 (C Terminus) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB06145 1
EB06145 (0.3µg/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06145 2
EB06145 (5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06145 3
EB06145 (5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB06145 4
EB06145 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: VPS45, VPS45A, VPS45B, VSP45, VSP45A, H1, H1VPS45, leucocyte vacuolar protein sorting 45, vacuolar protein sorting 45A, vacuolar protein sorting 45A (yeast homolog), vacuolar protein sorting 45 homolog (S. cerevisiae), vacuolar protein sorting 45A (yeast), vacuolar protein sorting 45B (yeast), vacuolar protein sorting protein similar to S. cerevisiae Vsp45p, RP11-458I7.2, VPS54A
Official Symbol: VPS45
Accession Number(s): NP_009190.2
Human GeneID(s): 11311
Non-Human GeneID(s): 22365 (mouse)

Immunogen

Peptide with sequence C-ESSQVTSRSASRR, from the C Terminus of the protein sequence according to NP_009190.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 60kDa band observed in Human Testis and Peripheral Blood Mononucleocyte lysates (calculated MW of 65.1kDa according to NP_009190.2). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cortex), Placenta and Small Intestine. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog, Cow

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