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Goat Anti-VEGFA (aa288-299) Antibody (EB11747)

Code Name Applications Availability Size Tested species Price Grade  
EB11747 Goat Anti-VEGFA (aa288-299) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $333.00 Elite

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ARP American Research Products, Inc
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EB11747 1
EB11747 (0.1µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11747 2
EB11747 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB11747 3
EB11747 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: MGC70609, MVCD1, OTTHUMP00000016487, OTTHUMP00000016488, OTTHUMP00000165985, OTTHUMP00000165986, OTTHUMP00000165987, OTTHUMP00000224107, OTTHUMP00000224108, OTTHUMP00000224109, OTTHUMP00000224153, OTTHUMP00000224154, OTTHUMP00000224423, OTTHUMP00000224424, OTTHUMP00000224425, OTTHUMP00000224426, OTTHUMP00000224427, OTTHUMP00000224428, OTTHUMP00000224429, OTTHUMP00000224430, vascular endothelial growth factor A, vascular permeability factor, VEGF, VEGFA, VPF
Official Symbol: VEGFA
Accession Number(s): NP_001020537.2; NP_003367.4; NP_001020538.2; NP_001020539.2; etc.
Human GeneID(s): 7422
Important Comments: This antibody is expected to recognize all reported isoforms.


Peptide with sequence C-RIKPHQGQHIGE, from the internal region of the protein sequence according to NP_001020537.2; NP_003367.4; NP_001020538.2; NP_001020539.2; etc..

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 45kDa band observed in lysates of cell line HEK293 (calculated MW of 45.5kDa according to NP_001020537.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat (Liver, Kidney and Lung) for this particular batch.
IHC: Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 5µg/ml.

Immunofluorescence: Testing has shown that this antibody stains mitochondria in Mouse NIH3T3 cells at 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Pig

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